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Title: Perbandingan Hasil Fertilisasi In Vitro (FIV) antara Sperma yang Berasal dari Ejakulat dengan Sperma yang Berasal dari Teknik Tesa/Mesa
Authors: Rudiyanto
Advisor: Kardhinata, E Harso
Nugrahalia, Meida
Keywords: fertilisasi in vitro;testicular sperm spiration;microsurgical epididymal sperm aspiration;ejakulat
Issue Date: 22-Apr-2017
Publisher: Universitas Medan Area
Abstract: Research on in vitro fertilization (FIV) in humans has been carried out to compare the success rate between sperm originating from ejaculate and sperm derived from Testicular Sperm Aspiration (TESA) and Microsurgical Epididymal Sperm Aspiration (MESA) techniques. The aim of the study was to analyze the comparison of successful pregnancy through FIV between sperm from TESA/ MESA results with sperm from ejaculate material. The research was conducted for 3 months at the Halim Fertility Center (HFC) Stella Maris Hospital, Medan and in the Division of Endocrinology and Infertility, Department of Obstetrics and Gynecology, Faculty of Medicine, University of North Sumatra. The samples in this study were all men from couples who participated in the FIV program. The research method used a completely randomized design and the data obtained were analyzed by ANOVA. The results showed ejaculation sperm motility was significantly higher (P <0.05) than sperm motility from TESA/MESA. The number of embryos transferred between the two groups was significantly different (P <0.05), but the percentage of pregnancies between the two groups showed not significantly different (P> 0.05).
Description: Penelitian tentang fertilisasi in vitro (FIV) pada manusia telah dilakukan untuk membandingkan tingkat keberhasilan antara sperma yang berasal dari ejakulat dengan sperma yang berasal dari teknik Testicular Sperm Aspiration (TESA) dan Microsurgical Epididymal Sperm Aspiration (MESA). Tujuan penelitian adalah untuk menganalisis perbandingan keberhasilan kehamilan melalui FIV antara sperma dari hasil TESA/MESA dengan sperma yang berasal dari bahan ejakulat. Penelitian dilakukan selama 3 bulan di Halim Fertility Center (HFC) Gedung RSIA Stella Maris, Medan serta di Divisi Endokrinologi dan Infertilitas, Departemen Obstetri dan Ginekologi, Fakultas Kedokteran, Universitas Sumatera Utara. Sampel penelitian ini adalah semua pria dari pasangan yang mengikuti program FIV. Metode penelitian menggunakan Rancangan Acak Lengkap dan data yang diperoleh dianalisis dengan ANOVA. Hasil penelitian menunjukkan motilitas sperma ejakulasi secara nyata lebih tinggi (P<0.05) dibandingkan motilitas sperma hasil TESA/MESA. Jumlah embrio yang ditranfer antara kedua kelompok adalah berbeda nyata (P<0.05), tetapi persentase kehamilan antara kedua kelompok menunjukkan hasil yang tidak berbeda nyata (P>0.05).
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